Stability studies and evaluation of the semi solid dosage form of the rutin, quercitin, ellagic acid, gallic acid and sitosterol isolated from the leaves of Tectona grandis for wound healing activity
نویسنده
چکیده
Gallic acid (GA), rutin(R), quercitin (Q), ellagic acid (EA) and sitosterol(S) isolated from the methanolic extract of the leaves of Tectona grandis were formulated as 0.2 % ointment in emulsifying base and were evaluated for their wound healing activity. The animals were divided into eight groups of six animals each. Groups 1 used as control, group 2 used as a standard i.e. nitrofurazone ointment (0.2 % w/w), group 3 for emulsifying ointment base, groups 4,5,6, 7 and 8 were used for gallic acid,rutin ,quercitin, ellagic acid and sitosterol . The formulation containing the most active phytoconstituent was packed in air tight bottle and evaluated for itsstability. The ointment was stored at 0 ,30, 45 at different predetermined intervals i.e. 30, 60 and 90 days and was evaluated for its appearance, spreadability, texture, pH and phase separation. The animals treated with rutin showed complete healing on the 10 day, followed by gallic acid, Ellagic acid, Sitosterol, Quercitin. The formulation containing rutin was subjected to stability testing .There was no change in color, pH and no phase separation at 0 and 30 for 90 days. The pH of the ointment was found to be in the range of 5.95-6.36 .The spreadability of the formulation was found to lie between 33.9252.77 and the spreading time ranges between 18 sec to 28 sec. The texture of the ointment remained smooth through out the stability studies. The drug content of the ointment was found with in the range of 100%-97.07% at 30.
منابع مشابه
Anti microbial and anti-oxidant properties of the isolated compounds from the methanolic extract from the leaves of Tectona grandis
The compounds Gallic acid (GA), rutin(R), quercitin (Q), ellagic acid (EA) and sitosterol(S) were isolated from the methanolic extract of the leaves of Tectona grandis. These compounds were subjected to antimicrobial and antioxidant activity. The zone of inhibition of isolated compounds was evaluated by cup plate method against bacteria i.e. Staphylococcus aureus, Bacillus subtilis, Eschericia ...
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